Therapeutic potential of mesenchymal stem cells tested in clinical and in vitro trials – the justification for stem cell banking
The aim of the project is to develop the use of adipose-derived stem cells (ADSC) in regenerative medicine. The project will include two types of research activity. Within “from laboratory to clinic” path we will use our knowledge and skills to prepare ADSC to clinical trials planned within the framework of the project: in the treatment of diabetic foot as well as aesthetic medicine (patients with scars and aging of the skin.) In “from laboratory to stem cell banking and innovative preparation of stem cells application” path we are planning to implement ADSC banking service involving individual characteristics of differentation potential of each deposited cell population.
We will search for the correlation between ADSC differentiation in stem cell culture and the results of transcriptomic and epigenomic analysis of cells as a quick method of evaluating the differentiation potential of the deposited cells. We will test new methods of preparing ADSC to application through the following techniques: mixed stem cells with different phenotypes derived from one population culture, connecting with biomaterials as cell carriers and “macromolecular crowding”. We will also test immunogenic character of human ADSC for the future use in GvHD (graft-versus-host disease) and in allogenic applications. The know-how generated by two academic centres will be validated and prepared to being implemented by four commercial partners.